Spectrophotometry method for titration of trout interferon, and its application to rainbow trout fry experimentally infected with viral haemorrhagic septicaemia virus
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Spectrophotometric method for titration of trout interferon, and its application to rainbow trout fry experimentally infected with viral haemorrhagic septicaemia virus
The activity of a reference preparation of rainbow trout circulating interferon (IFN), induced by experimental infection of fish with viral haemorrhagic septicaemia virus, was detected on the basis of its non-specific protective effect for RTG-2 cells, grown in microplates and challenged with infectious pancreatic necrosis virus. The IFN was titrated by spectrophotometric assessment of the abso...
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lmmunohistochem~stry and virus isolation were compared for their ability to detect viral haemorrhagic septicaemia vlrus (VHS) in experimentally ~nfected ralnbow trout Oncorhynchus mykiss. The f ~ s h were divided into 3 g]-oups ( I to I l l ) , and infected, respectively, by bath challenge w ~ t h 102, 103 ', and 10"CID50 ml-l water of a VHS virus strain serologically sllnilar to reference stra...
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Mammalian cytotoxic T cells as part of the adaptive immune system recognize virus-infected target cells by binding of their T-cell receptors (TCR) to classical MHC class I molecules loaded with viral peptides. Our previous studies have shown that the allele of the single dominant polymorphic classical MHC class I locus Onmy-UBA is identical in the rainbow trout clone C25 and in the permanent ra...
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Cells isolated from rainbow trout fins Oncorhynchus mykiss, Walbaum were propagated and subcultured a s fibroblast-like. Viral haemorrhagic septicaemia virus (VHSV) replicated in the cultured fin cells as demonstrated by fin cell lysis, posihve immunofluorescence with polyclonal antiVHSV, and Increase in titre as measured by either infection of f ~ s h cell lines or by sandwich ELISA with anti-...
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In general, viral haemorrhagic septicaemia virus (VHSV) isolates from marine fish species in European waters (genotypes GIb, GII and GIII) are non- to low virulent in rainbow trout. However, a VHSV isolation was made in 2007 from a disease outbreak in sea farmed rainbow trout in Norway. The isolate, named NO-2007-50-385, was demonstrated to belong to GIII. This isolate has attracted attention t...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 1991
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao010023